Exogenous expression of a histone H3.3 isoform causes extranuclear divisions in the <i>C. elegans</i> intestine.

ABSTRACT: Histone proteins condense DNA into chromatin and play significant roles in gene regulation. Mutations that alter histone function can disrupt critical gene expression programs and are implicated in driving cancer and other human diseases. Most mechanistic research on histones has been conducted in cell culture, mitotic tissues, or animal disease models rather than in normal tissue. Caenorhabditis elegans is a model organism with a well-established developmental program and conserved histone mechanisms shared with other metazoans. Prior work generated several single-copy histone reporters expressing exogenous histone protein in the intestine. Surprisingly, the histone H3.3 reporter worms possessed extra nuclei in the anterior intestine. This phenotype was incompletely penetrant and a

SUBMITTER: Sandoval CH 

PROVIDER: S-EPMC12047854 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature