Development and characterization of novel ELISAs for the specific quantification of the factor H-related proteins 2, 3, 4 and 5.

ABSTRACT:

Background

The complement system's alternative pathway relies on factor H (FH) for immune homeostasis. Next to FH, a group of highly similar proteins was described known as FH-related (FHR) proteins. The FH protein family includes FH, factor H-like protein 1, and five FHR proteins (FHR-1 to -5). The exact function of the FHRs is still unknown, necessitating further research. However, the lack of highly specific assays has hindered studying their role in health and disease. This study aimed to develop novel ELISAs for reliably and specifically quantifying levels of the FHRs in human blood.

Methods

Novel FHR specific antibodies were generated. Positive hybridoma clones were taken to monoclonality, verified for target specificity via ELISA and western blot, and antibody pairs w

SUBMITTER: van Rossum M 

PROVIDER: S-EPMC12048131 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature