The VEGF decoy receptor soluble Fms-like tyrosine kinase 1 binds to macrophages.

ABSTRACT:

Background

Soluble Fms-like Tyrosine kinase-1 (sFLT1) is a native inhibitor of VEGF, best known for its antiangiogenic effects in preeclampsia. sFLT1 also reduces chronic inflammation and promotes tissue repair. In experimental diabetic nephropathy, we previously found that sFLT1 ameliorates kidney fibrosis and reduces the infiltration of macrophages. How sFLT1 regulates inflammation is still incompletely understood. Based on the direct association of sFLT1 with various cell types, we here studied whether sFLT1 interacts with macrophages to modulate inflammation.

Methods

Using various macrophage cell lines, sFLT1 cell surface binding was detected with flow cytometry. Enzyme studies, mass spectrometry and RNAseq were employed to identify potential sFLT1 cell surface interacto

SUBMITTER: van Aanhold CCL 

PROVIDER: S-EPMC12048422 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature