The commercial PRRSV attenuated vaccine can be a potentially effective live trivalent vaccine vector.

ABSTRACT: PRRSV is an immunosuppressive virus that is prone to secondary infection by other viral and microbial pathogens, exacerbating its harm to pigs. The development of multivalent vaccines that can prevent and control multiple pathogens is of great significance for the pig industry. In order to evaluate the potential of commercial PRRSV vaccines as the viral vectors for the trivalent vaccine, this study first embedded mCherry or EGFP genes for fluorescent proteins into the infectious clone of the HP-PRRSV MLV vaccine strain GD to construct a recombinant strain. Furthermore, we constructed a recombinant PRRSV strain, rPRRSV-mCherry-EGFP, that expresses both EGFP and mCherry proteins simultaneously and evaluated the stability and immunogenicity of the exogenous proteins. The results showed that M

SUBMITTER: Li Y 

PROVIDER: S-EPMC12048423 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature