Genomic characterization and probiotic assessment of Bifidobacterium breve JKL2022 with strain-specific CLA-converting properties.

ABSTRACT: Bifidobacterium breve is a well-recognized probiotic species. B. breve JKL2022, a strain isolated from the feces of healthy infants that exhibits superior conjugated linoleic acid (CLA)-converting activity, was functionally characterized for probiotic safety and applicability through genomic and in vitro analyses. The JKL2022 genome comprises a 2,313,948 bp sequence assembled into a single contig, encoding a total of 1,998 genes. In silico predictive analyses confirmed the absence of virulence factors and acquired resistance genes while verifying its intrinsic antimicrobial resistance profile. Several CAZymes were identified, consistent with the strain's fermentation profile. Additionally, the gene encoding the key enzyme for CLA conversion was identified as a 993-bp lai gene, underscoring

SUBMITTER: Elnar AG 

PROVIDER: S-EPMC12048573 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature