Biomimetic culture substrates for modelling homeostatic intestinal epithelium in vitro.

ABSTRACT: The increasing interest in utilizing three-dimensional (3D) in vitro models with innovative biomaterials to engineer functional tissues arises from the limitations of conventional cell culture methods in accurately reproducing the complex physiological conditions of living organisms. This study presents a strategy for replicating the intricate microenvironment of the intestine by cultivating intestinal cells within bioinspired 3D interfaces that recapitulate the villus-crypt architecture and 3D tissue arrangement of the intestine. Intestinal cells cultured on these biomimetic substrates exhibited phenotypes and differentiation characteristics resembling intestinal-specific cell types, effectively replicating intestinal tissue. Notably, tissue proliferation and differentiation were achieved

SUBMITTER: Abdollahi S 

PROVIDER: S-EPMC12048609 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature