Precise modulation of protein refolding by rationally designed covalent organic frameworks.

ABSTRACT: Precisely regulating protein conformation (folding) for biomanufacturing and biomedicine is of great significance but remains challenging. In this work, we innovate a covalent organic framework (COF)-directed protein refolding strategy to modulate protein conformation by rationally designed covalent organic frameworks with adapted pore structures and customizable microenvironments. The conformation of denatured protein can be efficiently recovered through a simple one-step approach using covalent organic framework treatment in aqueous or buffer solutions. This strategy demonstrates high generality that can be applied to various proteins (for example, lysozyme, glucose oxidase, trypsin, nattokinase, and papain) and diverse covalent organic frameworks. An in-depth investigation of the refold

SUBMITTER: Guo J 

PROVIDER: S-EPMC12048718 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature