A rare peptide scaffold in kineomicins, the glycopeptide antibiotics produced by Actinokineospora auranticolor DSM 44650.

ABSTRACT: Discovery of novel antibiotics is crucial to counteract bacterial resistance spread. Aiming to expand the available arsenal of last-resort glycopeptide antibiotics (GPAs), we mined the actinobacterial genomes of Pseudonocardiales. We thus identified a biosynthetic gene cluster (BGC) encoding for a GPA with a novel peptide scaffold, not fitting into the existing classification of GPA types. By cultivating the producer strain, Actinokineospora auranticolor DSM 44650, an antibiotic complex-named kineomicins (Kmc)-was identified and characterized by microbiological assays, LC-MS, and MS/MS analyses. A comprehensive model for Kmc biosynthesis was then proposed by a thorough investigation of kineomicin BGC (knm). The structure of the main complex congener (KmcB), resolved by NMR spectroscopy, pr

SUBMITTER: Yushchuk O 

PROVIDER: S-EPMC12049478 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature