Delivery of small interfering RNA through lyophilized natural lipid nanoparticles: effects of natural lipid selection.

ABSTRACT:

Context

Lipid nanoparticles (LNPs) are the primary non-viral vectors for siRNA delivery. However, synthetic lipids face issues, such as low lysosomal escape efficiency and high cost.

Objective

This study aimed to use three natural lipids to construct LNPs, optimize their preparation and freeze-drying processes, and evaluate their siRNA delivery efficiency in vitro.

Materials and methods

Coix seed lipid [Coix lacryma-jobi L. var. mayuen (Roman.) Stapf (Poaceae), CSL], Brucea javanica seed lipid [Brucea javanica (L.) Merr. (Simaroubaceae), BJL], and Soybean oil [Glycine max (L.) Merr. (Fabaceae), SO] were used to construct LNPs. The Z-average size, zeta potential, Polymer Dispersity Index, and N/P ratio of the LNPs were c

SUBMITTER: Wang H 

PROVIDER: S-EPMC12051533 | biostudies-literature | 2025 Dec

REPOSITORIES: biostudies-literature