St. John's Wort Extract Increases Pgp Expression in the Brain but Not in the Small Intestine or the Liver of Wistar Rats.

ABSTRACT: St John's Wort (SJW), commonly used to treat mild depression, is known to pose a risk of drug-herb interactions through hyperforin-mediated activation of the pregnane X receptor (PXR). This induces transcription and expression of PXR target genes, including the efflux transporter P-glycoprotein (Pgp). While the activation of human PXR by the SJW constituent hyperforin is well established, there are contradictory findings on rodent PXR target genes. This study aimed to further investigate SJW effects on Pgp expression in rats. Male Wistar rats were treated for 10 days with the two commercial SJW formulations, Hyperiplant and Rebalance, which differ in their hyperforin content. Quantitative real-time PCR, western blot analysis, and immunohistochemical staining were applied to test for Pgp mR

SUBMITTER: Taggi V 

PROVIDER: S-EPMC12052523 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature