Genetic modification of Water spinach (Ipomoea aquatica), a genoprotective perennial leafy green.

ABSTRACT: Improvement of leafy greens, especially perennials with year-round harvesting, is binding to the food security drive. "Food for All" by WHO demands the improvement of regional crops due to the agroclimatic specificity to ensure regional food security. Water spinach (Ipomoea aquatica) is a perennial nutritious leafy green with regional/ethnic cultivation. We accomplished organogenesis and somatic embryogenesis from different explants of I. aquatica, and transgenesis and genome editing through Agrobacterium-mediated transformation. The Ipomoea Basal (CLC-CP) medium was superior to the Murashige and Skoog medium. Hypocotyl explants produced a mean of 12.4 shoots on CLC-CP containing 4.5 μM thidiazuron and 8.7 μM gibberellic acid (GA₃), 50 mg l^-1 ascorbic acid (AA), and 1

SUBMITTER: Purayil FT 

PROVIDER: S-EPMC12052931 | biostudies-literature | 2025 May-Jun

REPOSITORIES: biostudies-literature