Cancer-Specific RNA Modifications in Tumour-Derived Extracellular Vesicles Promote Tumour Growth.

ABSTRACT: RNA modifications are crucial in cellular processes, and their dysregulation is linked to diseases like cancer. Extracellular vesicles (EVs) contain various RNAs and might be susceptible to modifications, but detecting these modifications has been challenging due to the small amount of RNA in EVs. We successfully detected 22 RNA modifications in EVs using a proprietary ultra-HPLC MS/MS system. We identified reduced levels of N6-methyladenosine (m6A) in EVs derived from colon cancer tissues, which correlated with cancer recurrence. Increasing m6A levels via m6A demethylase Alkbh5 knockout suppressed the tumour-promoting effects of colorectal cancer EVs. Mechanistically, colorectal cancer-derived EVs increased tumour necrotic factor α and interleukin-6 secretion by macrophages via Toll-like

SUBMITTER: Monoe Y 

PROVIDER: S-EPMC12053886 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature