Identification of essential genes by transposon insertion sequencing and genome-scale metabolic model construction in <i>Streptococcus suis</i>.

ABSTRACT: Bacterial essential genes are indispensable for the survival of bacteria and therefore are attractive targets for novel anti-microbial drugs. Identifying essential genes provides a roadmap for developing novel antibiotics and anti-microbial therapies. In this study, combining high-throughput transposon sequencing (Tn-seq) and genome-scale metabolic model (GEM) construction, essential genes of Streptococcus suis, an important emerging zoonotic bacterial pathogen, were analyzed. A highly efficient transposon (Tn) mutagenesis system was developed in S. suis. This system facilitated the construction of a high-density library containing over 160,000 Tn mutants. By sequencing the library and data analysis, more than 21,000 insertion sites and 150 essential genes for growth in the r

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC12053914 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature