CAMP-negative <i>Streptococcus agalactiae</i> strains exhibited complete or partial chromosomal deletions of the CAMP-factor encoding gene <i>cfb</i>.

ABSTRACT: Universal antepartum group B streptococcus (GBS) screening and intrapartum antibiotic prophylaxis (IAP) have effectively reduced early-onset GBS infections. However, GBS strains with chromosomal deletions affecting the cfb gene may produce false negatives in both the CAMP test and cfb-based molecular diagnostics, potentially increasing the risk of neonatal infections. Vaginal swabs were collected from pregnant women at 35-37 weeks of gestation in our hospital and cultured on agar. Suspected GBS strains were initially identified using the CAMP test and then confirmed with the VITEK-2 system. CAMP-negative GBS strains underwent additional testing by qPCR, 16S rDNA, serotyping, and multilocus sequence typing (MLST). PCR for the cfb gene and whole-genome sequencing were pe

SUBMITTER: Lai X 

PROVIDER: S-EPMC12054153 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature