Structural insights into thraustochytrid-specific lipases using alphafold to identify the role of GXSXG motif.

ABSTRACT:

Background

Triacylglycerol lipases (E.C. 3.1.1.3) are serine hydrolases, universally present in animals, plants and microbes and are an integral part of lipid metabolism. They are industrially relevant enzymes that cleave ester bonds of triacylglycerides to release free fatty acids and glycerol. Thraustochytrid Aurantiochytrium limacinum SR21 has previously been reported to utilize 120 g L^- 1 of oil substrate. Previously, thraustochytrid specific lipases was reported that allowed the microbe to thrive on oil substrate, however the structural characteristics of these enzymes remain undetermined.

Results

In this study, we identified nearly 30 genes that encode TAG lipases with Lipase_3 domain, allowing the marine microbe to thrive on oil substrate. The lipases were

SUBMITTER: Mariam I 

PROVIDER: S-EPMC12054267 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature