Clusterin ameliorates diabetic atherosclerosis by suppressing macrophage pyroptosis and activation.

ABSTRACT:

Background

It has been demonstrated that clusterin (CLU) is a protective protein involved in a variety of diseases and disorders. However, the role of CLU in diabetic atherosclerosis is not elucidative. The objective of this study is to investigate the role of CLU in diabetic atherosclerosis and the molecular mechanisms.

Method

In in vivo experiments, Clu knockout and overexpressed murine models were used to investigate the role of Clu in diabetic atherosclerosis. Atherosclerotic plaque formation was determined by hematoxylin-eosin (H&E) staining and Oil Red O staining. F4/80 and CD68 levels were determined by immunohistochemical staining. Transmission electron microscopy was used to observe changes in cell pyroptosis morphology. NLRP3 and IL-1β levels were det

SUBMITTER: Xuan L 

PROVIDER: S-EPMC12055819 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature