Differential reactivity of SARS-CoV-2 S-protein T-cell epitopes in vaccinated versus naturally infected individuals.

ABSTRACT:

Objectives

Vaccine-induced protective immunity against SARS-CoV-2 has proved difficult to sustain. Robust T-cell responses are thought to play an important role, but T-cell responses against the SARS-CoV-2 spike protein (S-protein), the core vaccine antigen, following vaccination or natural infection are incompletely understood.

Methods

Herein, the reactivity of 170 putative SARS-CoV-2 S-protein CD8⁺ and CD4⁺ T-cell peptide epitopes in the same individuals prior to vaccination, after COVID-19 vaccination, and again following subsequent natural infection was assayed using a high-throughput reverse transcription-quantitative PCR (HTS-RT-qPCR) assay.

Results

The profile of immunoreactive SARS-CoV-2 S-protein epitopes differed between vaccination and

SUBMITTER: Browne DJ 

PROVIDER: S-EPMC12056234 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature