Identification of autoantibodies targeting citrullinated CLEC12A in rheumatoid arthritis patients.

ABSTRACT:

Objective

Rheumatoid arthritis is an autoimmune disease characterized by anti-citrullinated protein antibodies (ACPA). The pathogenic and protective roles of ACPA of distinct specificities are emerging and remains poorly understood. Thus, it is crucial to define the range of ACPA specificities and determine their contribution to disease and their potential clinical relevance. Since extracellular citrullination occurs in RA, we investigated whether autoantibodies in RA patients bind a citrullinated form of the cell-surface receptor CLEC12A that is expressed on neutrophils, the most abundant leukocyte in inflamed joints.

Methods

We generated a FLAG-tagged, recombinant form of the extracellular portion of human CLEC12A. After purification, the tag was removed prior to citrullin

SUBMITTER: Barra L 

PROVIDER: S-EPMC12056953 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature