Identification and characterization of a novel major facilitator superfamily (MFS) efflux pump conferring multidrug resistance in <i>Staphylococcus aureus</i> and <i>Staphylococcus epidermidis</i>.

ABSTRACT: A novel major facilitator superfamily (MFS) efflux pump in Staphylococcus, designated Nms, was identified via topology prediction. The secondary structure indicated the presence of 12 transmembrane segments (TMSs) and characteristic motif A of MFS efflux pumps. Experimental verification of efflux activity was conducted using ethidium bromide accumulation and efflux assays and biofilm formation assays. Antimicrobial susceptibility testing and efflux pump inhibition confirmed that Nms effectively effluxed various antimicrobial agents to confer multidrug resistance. Comprehensive genomic analyses were used to assess the prevalence and possible origins of the nms gene. The results revealed that the nms gene was present in Staphylococcus aureus ST398/ST541 and Sta

SUBMITTER: Huang H 

PROVIDER: S-EPMC12057375 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature