Microphysiological gut-on-chip enables extended <i>in vitro</i> development of <i>Cryptosporidium hominis</i>.

ABSTRACT:

Introduction

Cryptosporidium hominis is the dominant Cryptosporidium species infecting humans, but most advances in developing robust in vitro culturing platforms for Cryptosporidium have utilised C. parvum. Consequently, there is relatively little available information specific to the biology and life cycle of C. hominis. The present study utilised a pumpless and tubeless gut-on-chip to generate a physiologically relevant in vitro environment by applying a constant fluid shear stress of 0.02 dyn cm^-2 to HCT-8 cells.

Methods

Gut-on-chips were fabricated using standard soft lithography. C. hominis oocysts isolated from human pathology samples were used to infect the human ileocecal colorectal adenocarcinoma (H

SUBMITTER: Gunasekera S 

PROVIDER: S-EPMC12058726 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature