Association mapping and identification of candidate genes for callus induction and regeneration using sorghum mature seeds.

ABSTRACT:

Introduction

A whole plant can be regenerated through tissue culture from an embryogenic callus in a process referred to as plant regeneration. Regeneration ability of embryogenic callus is a quantitative trait and the main limiting factor for genetic studies in sorghum.

Methods

We evaluated 236 sorghum mini core varieties for callus induction rate, embryogenic callus rate, callus browning rate and differentiation rate and performed a multi-locus genome-wide association study (GWAS) of the four traits with 6,094,317 SNPs.

Results

We found five mini core varieties most amenable to tissue culture manipulations: IS5667, IS24503, IS8348, IS4698, and IS5295.Furthermore, we mapped 34 quantitative trait loci (QTLs) to the four traits and identified 47 candidate genes. Previo

SUBMITTER: Xu J 

PROVIDER: S-EPMC12058750 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature