PDGF-BB overexpressing dental pulp stem cells improve angiogenesis in dental pulp regeneration.

ABSTRACT:

Introduction

Angiogenesis represents a critical challenge in dental pulp regeneration due to the tissue's restricted nutrient supply through a 0.5-mm apical foramen. While dental pulp stem cells (DPSCs) hold regenerative potential, their limited vascularization capacity impedes clinical applications. Through Single-cell RNA sequencing (scRNA-seq) analysis of human dental pulp, we discovered a PDGF (+) mesenchymal subset exhibiting enhanced angiogenic signatures, suggesting targeted cell selection could overcome this bottleneck.

Methods

ScRNA-seq identified PDGF (+) subpopulation in human pulp samples, validated through multiplex immunohistochemical of the localization of PDGF/CD73/CD31. PDGF-BB-overexpressing DPSCs were engineered via lentiviral vectors. Functional assessmen

SUBMITTER: Jiang W 

PROVIDER: S-EPMC12058851 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature