Role of Raf Kinase Inhibitor Protein in Modulating Echinococcal Cyst Fluid-Induced Degranulation in Bone Marrow-Derived Mast Cells.

ABSTRACT:

Introduction: This study aimed to investigate the role of Raf kinase inhibitor protein (RKIP) in degranulation induced by echinococcal cyst fluid (EgCF) in bone marrow-derived mast cells (BMMCs).

Methods

Primary BMMCs were isolated and cultured from the femurs and tibias of RKIP gene knockout (KO) and wild-type (WT) C57BL/6 mice. EgCF-induced degranulation models were established for both groups. Samples of cells and supernatant were collected for analysis. Surface expression levels of CD117 and Fc-epsilon Receptor Ⅰ α (FcεRIα) were assessed. Supernatant concentrations of β-hexosaminidase, IL-4, IL-6, and tumour necrosis factor (TNF-α) were measured. Cellular mRNA levels of IL-4, IL-6, and TNF-α were quantified, and changes in RKIP protein expression during degranulation in WT BM

SUBMITTER: Ye JR 

PROVIDER: S-EPMC12060804 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature