Development of genome-wide SSR markers through <i>in silico</i> mining of guava (<i>Psidium guajava</i> L.) genome for genetic diversity analysis and transferability studies across species and genera.

ABSTRACT: Guava (Psidium guajava L.) is one of the economically major fruit crops, abundant in nutrients and found growing in tropical-subtropical regions around the world. Ensuring sufficient genomic resources is crucial for crop species to enhance breeding efficiency and facilitate molecular breeding. However, genomic resources, especially microsatellite or simple sequence repeat (SSR) markers, are limited in guava. Therefore, novel genome-wide SSR markers were developed by utilizing chromosome assembly (GCA_016432845.1) of the "New Age" cultivar through GMATA, a comprehensive software. The software evaluated about 397.8 million base pairs (Mbp) of the guava genome sequence, where 87,372 SSR loci were utilized to design primers, ultimately creating 75,084 new SSR markers. After in silico

SUBMITTER: Pramanik K 

PROVIDER: S-EPMC12062180 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature