Analysis of biofilm assembly by large area automated AFM.

ABSTRACT: Biofilms are complex microbial communities critical in medical, industrial, and environmental contexts. Understanding their assembly, structure, genetic regulation, interspecies interactions, and environmental responses is key to developing effective control and mitigation strategies. While atomic force microscopy (AFM) offers critically important high-resolution insights on structural and functional properties at the cellular and even sub-cellular level, its limited scan range and labor-intensive nature restricts the ability to link these smaller scale features to the functional macroscale organization of the films. We begin to address this limitation by introducing an automated large area AFM approach capable of capturing high-resolution images over millimeter-scale areas, aided by machi

SUBMITTER: Millan-Solsona R 

PROVIDER: S-EPMC12062311 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature