Selective TCRαβ+ and CD45RA+ T-cell depletion of hematopoietic stem cell graft: An analysis on factors that affect depletion performance.

ABSTRACT: Selective depletion of TCRαβ+ and CD45RA+ subsets of apheresed hematopoietic progenitor cells, HPC(A), enables haploidentical hematopoietic stem cell transplant (haplo-HSCT) by circumventing risks of graft-versus-host disease. Here, we analyze our institution's large series of ex vivo T-cell depletion processes to review procedure performance and explore factors that affect depletion efficiency and graft composition. Over 6 years, 91 haploidentical donors underwent peripheral blood CD34+ stem cell mobilization with granulocyte-colony stimulating factor, with 12 (13%) receiving additional pre-emptive plerixafor. HPC(A) was split into two fractions for TCRαβ and CD45RA depletion with the CliniMACS PLUS device. TCRαβ depletion resulted in a median 4.3 (interquartile range, 4.1-4.5) log

SUBMITTER: Ang CH 

PROVIDER: S-EPMC12062598 | biostudies-literature | 2025 Jan-Dec

REPOSITORIES: biostudies-literature