Targeted activation of junctional adhesion molecule-like protein<b>+</b> CD8<b>+</b> T cells enhances immunotherapy in hepatocellular carcinoma.

ABSTRACT:

Objective

Cytotoxic T lymphocytes (CTLs) play a crucial role in the therapeutic approach to hepatocellular carcinoma (HCC). Recent research has indicated that junctional adhesion molecule-like protein (JAML) enhances the antitumor activity of CD8+ T cells. Our study investigates the role of JAML+ CD8+ T cells in HCC.

Methods

We utilized time-of-flight mass cytometry and an orthotopic mouse model of HCC to examine histone modifications in tumor-infiltrating immune cells undergoing immunotherapy. Flow cytometry was used to assess CD4+ T cells differentiation and JAML expression in CD8+ T cells infiltrating HCC. Correlation analysis revealed a strong positive correlation between lactate dehydrogenase A+ (LDHA+) CD4+ T cells and JAML+ CD8+ T cells. Subsequently, we evaluated the

SUBMITTER: Chen H 

PROVIDER: S-EPMC12062980 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature