Polymicrobial detection and salivary metabolomics of children with early childhood caries.

ABSTRACT:

Background

Early childhood caries (ECC) has been proposed to be associated with various microorganisms and metabolites. This study aims to compare the prevalence of specific microbial species and salivary metabolomics profile in children with and without ECC, and to explore the correlation between salivary metabolites and targeted microbes.

Method

Five ml of unstimulated saliva was collected from 32 ECC and 22 caries-free children. Clinical indexed were recorded and questionnaires regarding oral health and dietary habits were obtained from the guardians. The presence of eight specific microbial species were examined using species-specific quantitative PCR (qPCR). Untargeted metabolomics was analyzed to identify key differential metabolites and pathways. Correlations among cl

SUBMITTER: Pan T 

PROVIDER: S-EPMC12063607 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature