Matching plasma and tissue miRNA expression analysis to detect viable ovarian germ cell tumors.

ABSTRACT:

Purpose

MicroRNAs (miRNAs) are emerging as circulating biomarkers in germ cell tumors (GCT) with potential to guide management. Their role and expression patterns are more established in testicular GCTs, while lesser data exist in ovarian GCTs (OGCT).

Methods

Patients diagnosed with OGCT with plasma and tumor tissue available in our provincial biobank were included. Total RNA was extracted, and RT-qPCR was performed to measure miR-371-3 and miR-302/367 levels. Healthy plasma and ovarian tissue served as controls. Statistical analyses were performed using ANOVA and the Mann-Whitney U test. Clinicopathologic data was collected by chart review.

Results

From 2007 to 2022, 23 patients with OGCT were identified: 13 with viable non-teratoma germ cell (VNTGC) and 10 with imma

SUBMITTER: Angeles A 

PROVIDER: S-EPMC12063854 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature