Expression of HIV envelope protein in the human central nervous system.

ABSTRACT:

Objective/design

The HIV envelope glycoprotein gp120 contributes to neuronal damage that could lead to HIV-mediated neurocognitive disorder. However, it is debated whether gp120 could promote direct neurotoxicity to synapses as it may not be present in the central nervous system of people with HIV (PWH) on antiretroviral therapy (ART). This study sought to establish whether gp120 is expressed in the human central nervous system in the pre-ART and post-ART era.

Methods

We utilized real-time PCR to detect the envelope mRNA in postmortem caudate nucleus from pre-ART era. HIV-negative samples were used as controls. We then used RNAscope fluorescence multiplex to identify which cells express the envelope mRNA. To determine whether gp120 is present despite ART, we analyzed cerebro

SUBMITTER: Agbey C 

PROVIDER: S-EPMC12064364 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature