Enhancement of iminosugar production, 1-deoxynojirimycin and 1-deoxymannojirimycin, in recombinant <i>Corynebacterium glutamicum</i>.

ABSTRACT: The iminosugars 1-deoxynojirimycin (1-DNJ) and 1-deoxymannojirimycin (1-DMJ) were produced by recombinant Corynebacterium glutamicum (CgTYB) bearing the 1-DNJ-producing GabT1-Yktc1-GutB1 (TYB) gene cluster from Bacillus velezensis MBLB0692. The enhanced iminosugar biosynthesis in CgTYB cultures increased both α-mannosidase inhibition (AMI) and α-glucosidase inhibition (AGI) activity. Individual cultures harboring GabT1, Yktc1, and GutB1 genes were found to be able to produce both 1-DNJ and 1-DMJ in mixed cultures of one pot analysis. This suggests that the genes necessary for epimerization and reduction are intrinsic to the cells rather than close to the 1-DNJ-producing cluster. Glucose was the preferred carbon source and a provision of 10 g/L glucose increased

SUBMITTER: Siziya IN 

PROVIDER: S-EPMC12064549 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature