Mapping the molecular signature of ABA-regulated gene expression in germinating barley embryos.

ABSTRACT:

Background

Abscisic acid (ABA) regulates key plant processes, including seed germination, dormancy, and abiotic stress responses. While its physiological role in germination is well-documented, the molecular mechanisms are still poorly understood. To address this, we analyzed transcriptomic and metabolomic changes in ABA-treated germinating barley (Hordeum vulgare) embryos. To map ABA-responsive gene expression across embryonic tissues, we employed the Visium Spatial Transcriptomics (10× Genomics). This approach, which remains technically challenging to be applied in plant tissues, enabled the precise localization of gene expression across six embryo regions, offering insights into tissue-specific expression patterns that cannot be resolved by traditional RNA-seq.

Results

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SUBMITTER: Sybilska E 

PROVIDER: S-EPMC12065168 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature