Thrombospondin-1 induces CD8⁺ T cell exhaustion and immune suppression within the tumor microenvironment of ovarian cancer.

ABSTRACT:

Background

Ovarian cancer (OC) progression is heavily influenced by the tumor microenvironment (TME), where immune suppression plays a critical role. This study explores the role of thrombospondin-1 (THBS1) in regulating tumor-associated macrophages (TAMs), T cell exhaustion, and immune checkpoint expression, as well as its transcriptional regulation by SNF2H.

Methods

We analyzed THBS1 expression and its clinical significance using publicly available datasets (TCGA-OV, GSE14407) and tissue microarrays containing OC and adjacent normal tissues. In vitro functional studies were conducted using OC cell lines (SKOV3, A2780) and co-cultures with macrophages. Chromatin immunoprecipitation (ChIP) assays and RNA interference were employed to investigate SNF2H-mediated transcriptiona

SUBMITTER: Liang H 

PROVIDER: S-EPMC12065243 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature