Colora: a Snakemake workflow for complete chromosome-scale de novo genome assembly.

ABSTRACT:

Motivation

De novo assembly creates reference genomes that underpin many modern biodiversity and conservation studies. Large numbers of new genomes are being assembled by labs around the world. To avoid duplication of efforts and variable data quality, we desire a best-practice assembly process, implemented as an automated portable workflow.

Results

Here, we present Colora, a Snakemake workflow that produces chromosome-scale de novo primary or phased genome assemblies complete with organelles using Pacific Biosciences HiFi, Hi-C, and optionally Oxford Nanopore Technologies reads as input. Colora is a user-friendly, versatile, and reproducible pipeline that is ready to use by researchers looking for an automated way to obtain high-quality de novo genome assemblies.

Availab

SUBMITTER: Obinu L 

PROVIDER: S-EPMC12065627 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature