Native proteins from Galdieria sulphuraria to replace fetal bovine serum in mammalian cell culture.

ABSTRACT: The use of fetal bovine serum (FBS) in cell culture applications causes high costs and unacceptable animal suffering when FBS is extracted from fetal calves. Despite efforts, the exact composition of FBS still remains partially unresolved. Native proteins in FBS, such as growth factors, and their binding to cell receptors seem to be crucial for cell proliferation and differentiation. Recently, algal extracts with high protein content were considered to reduce the FBS demand. Algae extracts yielded promising results as growth serum in mammalian cell culture. Nevertheless, the dependence on residual FBS and the undefined composition of algae extracts are challenges. In this study, we aimed to yield highly concentrated extracts of native proteins from mixotrophically grown Galdieria sulphurar

SUBMITTER: Eisenberg H 

PROVIDER: S-EPMC12065734 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature