Engineering an Overflow-Responsive Regulation System for Balancing Cellular Redox and Optimizing Microbial Production.

ABSTRACT: Escherichia coli accumulates acetate as a byproduct in fast growth aerobic conditions when using glucose as carbon source. This phenomenon, known as overflow metabolism, has negative impacts on cell growth and protein expression, also causes carbon loss during biosynthesis in most microbial production scenarios. In this study, we regarded the "waste" metabolite as a useful metabolism indicator, constructed an overflow biosensor to monitor the change of acetate concentration and converted the signal into various regulation outputs. Phloroglucinol is a phenolic compound with several derivatives that exhibit various pharmacological activities. By applying the bifunctional dynamic regulation system on the phloroglucinol production, we released the cellular redox pressure in real-time and reduc

SUBMITTER: Zhang J 

PROVIDER: S-EPMC12067040 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature