Nonredundant Role of Leishmanolysin-Like (Lmln) Zinc-Metallopeptidase in Retinal Homeostasis.

ABSTRACT:

Purpose

To determine if Lmln, a Zinc-metallopeptidase, is important for retinal homeostasis.

Design

Basic research in mouse models of retinal degeneration.

Methods

Combining an unbiased N-ethyl-N-nitrosourea mutagenesis pipeline in mice with optical coherence tomography (OCT) screening and automated meiotic mapping, we identified an allele (nemeth) that seemed to be associated with outer nuclear layer (ONL) thinning. Since nemeth was predicted to lead to a nonsense mutation of the Lmln gene, we targeted Lmln using CRISPR/Cas-9 technology and characterized the impact on retinal anatomy and function.

Results

OCT imaging demonstrated an outer retinal degeneration in Lmln^-/- mice (P = 7.3 × 10^-9 for ONL at 2 m) that progressed over the first 6

SUBMITTER: Ufret-Vincenty RL 

PROVIDER: S-EPMC12067076 | biostudies-literature | 2025 Jan

REPOSITORIES: biostudies-literature