Probing the ferredoxin:hydrogenase electron transfer complex by infrared difference spectroscopy.

ABSTRACT: Ferredoxins are small iron-sulfur proteins that engage in one-electron transfer with oxidoreductases across all domains of life. The catalyzed reactions often include multiple electrons, e.g., in the two-electron reduction of NADP⁺ during photosynthesis or the reduction of protons to H₂ by the metalloenzyme hydrogenase. To date, the microscopic details of how ferredoxins facilitate multiple electron redox chemistry are unknown. Ferredoxins of the Allochromatium vinosum subfamily contain two [4Fe-4S] clusters, which allows for two one-electron transfer reactions. However, the iron-sulfur clusters of conventional 2[4Fe-4S]-type ferredoxins have very similar reduction potentials and conclusive evidence for the transfer of two electrons during a single prote

SUBMITTER: Sahin S 

PROVIDER: S-EPMC12067460 | biostudies-literature | 2025 Jun

REPOSITORIES: biostudies-literature