Tick feeding or vaccination with tick antigens elicits immunity to the <i>Ixodes scapularis</i> exoproteome in guinea pigs and humans.

ABSTRACT: Ixodes scapularis is a primary vector of tick-borne pathogens in North America. Repeated exposure to these ticks can induce a humoral response to tick antigens and acquired tick resistance. However, identifying antigens contributing to this resistance is challenging because of the vast number of I. scapularis proteins secreted during feeding. To address this, we developed I. scapularis rapid extracellular antigen monitoring (IscREAM), a technique to detect antibody responses to more than 3000 tick antigens. We validated IscREAM with immunoglobulin G (IgG) from guinea pigs vaccinated with tick antigens, including a cement antigen cocktail that induced tick resistance. Furthermore, we explored the natural response to tick bites by profiling antigens recogni

SUBMITTER: Hart TM 

PROVIDER: S-EPMC12067475 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature