A transgenic mouse line for rabies virus-mediated trans-synaptic tracing in the postnatal developing brain.

ABSTRACT: Neural circuits are composed of numerous neurons that perform diverse functions. Understanding the mechanisms of neural processing requires elucidating the connections among individual neurons. Rabies virus (RV)-mediated trans-synaptic tracing enables the visualization of direct presynaptic neurons of a defined neural population, facilitating the precise mapping of neural circuits across various brain regions. This method relies on RV mutants that require the expression of the TVA receptor and rabies glycoprotein to infect and spread to presynaptic neurons. Traditionally, adeno-associated virus (AAV) has been used to express these proteins. However, because AAV requires several weeks to achieve sufficient gene expression, it is challenging to use this approach for studying neural connectio

SUBMITTER: Inada K 

PROVIDER: S-EPMC12068592 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature