Reproducibility of in vivo electrophysiological measurements in mice.

ABSTRACT: Understanding brain function relies on the collective work of many labs generating reproducible results. However, reproducibility has not been systematically assessed within the context of electrophysiological recordings during cognitive behaviors. To address this, we formed a multi-lab collaboration using a shared, open-source behavioral task and experimental apparatus. Experimenters in 10 laboratories repeatedly targeted Neuropixels probes to the same location (spanning secondary visual areas, hippocampus, and thalamus) in mice making decisions; this generated a total of 121 experimental replicates, a unique dataset for evaluating reproducibility of electrophysiology experiments. Despite standardizing both behavioral and electrophysiological procedures, some experimental outcomes were hi

SUBMITTER: International Brain Laboratory 

PROVIDER: S-EPMC12068871 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature