Characterizing spatial immune architecture in metastatic melanoma using high-dimensional multiplex imaging.

ABSTRACT:

Introduction

Immune checkpoint inhibitors (ICIs) have significantly improved survival for patients with metastatic melanoma, yet many experienceresistance due to immunosuppressive mechanisms within the tumor immune microenvironment (TIME). Understanding how the spatial architecture of immune and inflammatory components changes across disease stages may reveal novel prognostic biomarkers and therapeutic targets.

Methods

We performed high-dimensional spatial profiling of two melanoma tissue microarrays (TMAs), representing Stage III (n = 157) and Stage IV (n = 248) metastatic tumors. Using imaging mass cytometry (IMC) and multiplex immunofluorescence (mIF), we characterized the phenotypic, functional, and spatial properties of the TIME. Cellular neighborhoods wer

SUBMITTER: Eliason J 

PROVIDER: S-EPMC12069457 | biostudies-literature | 2025

REPOSITORIES: biostudies-literature