Evaluation of the Alterations in Central Cholinergic Neurotransmission in Aging and Amyloid Precursor Protein Knock-In Mice.

ABSTRACT: A progressive decline in cognitive function occurs as a result of aging and Alzheimer's disease (AD) and is primarily associated with diminished cholinergic neurotransmission. However, the precise mechanisms contributing to cholinergic dysfunction are not fully elucidated. Herein, we evaluated the cholinergic system in wild type (WT) mice and AD-model (App^NL-G-F) mice exhibiting overproduction of amyloid-beta 42 (Aβ₄₂). In superfusion experiments, [³H]acetylcholine (ACh) release from the frontal cortex and hippocampal segments preloaded with [³H]choline exhibited no significant differences between adult (6-8 months old) and aged (12-17 months old) WT mice. Uptake of [³H]choline via the high-affinity choline transporter 1 (CHT1) and the

SUBMITTER: Nagai-Arakawa I 

PROVIDER: S-EPMC12070134 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature