Utilizing Nanopore direct RNA sequencing of blood from patients with sepsis for discovery of co- and post-transcriptional disease biomarkers.

ABSTRACT:

Background

RNA sequencing of whole blood has been increasingly employed to find transcriptomic signatures of disease states. These studies traditionally utilize short-read sequencing of cDNA, missing important aspects of RNA expression such as differential isoform abundance and poly(A) tail length variation.

Methods

We used Oxford Nanopore Technologies sequencing to sequence native mRNA extracted from whole blood from 12 patients with definite bacterial and viral sepsis and compared with results from matching Illumina short-read cDNA sequencing data. Additionally, we explored poly(A) tail length variation, novel transcript identification, and differential transcript usage.

Results

The correlation of gene count data between Illumina cDNA- and Nanopore RNA-sequencing st

SUBMITTER: He J 

PROVIDER: S-EPMC12070577 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature