<i>MBOVJF4278_00820</i> encodes a novel cytoadhesin of <i>Mycoplasma bovis</i> binding to heparin.

ABSTRACT: Mycoplasma bovis, a minimal bacterium but a notorious cattle pathogen, leads to serious economic losses. This pathogen binding to host cells is emerging as a complex process involving a broad range of surface-exposed structures. For mycoplasma, adhering to host tissue is the first and crucial step of infection. It is well known that the molecules contributing to microbial cytoadhesion are important virulence factors. Here, we cloned, expressed, and purified the recombinant protein, which is encoded by MBOVJF4278_00820, and induced polyclonal antibodies for it in mice. The cytoadhesive properties of this surface-exposed protein were demonstrated on embryonic bovine lung (EBL) and Madin-Darby bovine kidney (MDBK) cells. Furthermore, heparin as the binding target was confirmed,

SUBMITTER: Wu Q 

PROVIDER: S-EPMC12070734 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature