Comparative Proteome and Weighted Gene Co-Expression Network Analyses Uncover the Mechanism of Wheat Grain Protein Accumulation in Response to Nitrogen Fertilizer Application.

ABSTRACT: This study uses proteomic technology to identify differentially expressed proteins (DEPs) under varying nitrogen fertilizer levels. Additionally, it utilizes weighted gene co-expression network analysis (WGCNA) based on expression data of DEP-coding genes to explore the mechanism by which nitrogen promotes grain protein accumulation. The results indicate that high-nitrogen treatment leads to an increased grain protein content, wet gluten content, stability time, and energy area. In addition, the β-sheet content of the protein secondary structure increased, while the irregular curl content decreased. A total of 285 DEPs were identified under different nitrogen levels, with 172 upregulated proteins in grains under high-nitrogen treatment including storage proteins (8.14%) and proteins involv

SUBMITTER: Xu B 

PROVIDER: S-EPMC12071265 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature