Remote precursor elements can modulate RNA induced silencing complex-loading efficiency of miR168 in Arabidopsis.

ABSTRACT: RNA interference mediated via the action of micro RNAs (miRNAs) plays a pivotal role in developmental and stress response pathways. In the nucleus, plant miRNAs are generated by subsequent enzymatic cuts of the MIRNA precursors, having specific hairpin-like secondary structures, to liberate the miRNA/miRNA* duplex. The mature miRNA strands are then loaded mostly into the ARGONAUTE 1-containing RNA induced silencing complex (RISC) with various efficiencies and trigger the down-regulation of the expression of the target mRNAs, while the miRNA* strands are eliminated. Here we revealed that MIRNA precursor structural elements, not overlapping with the miRNA/miRNA* duplex part, can have an influence on the AGO-loading efficiency of the produced miRNAs. Using transient and transgenic expression

SUBMITTER: Dalmadi A 

PROVIDER: S-EPMC12071341 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature