Ultrasound-Guided Rete Testis Approach to Sperm Aspiration and Spermatogonial Stem Cell Transplantation in Patients with Azoospermia.

ABSTRACT:

Background

Azoospermia, characterized by the absence of sperm in the ejaculate, impacts 1% of men globally. Surgical approaches to retrieve sperm from the testis are effective in some cases but can be invasive and expensive. Here we described a noninvasive ultrasound-guided rete testis (UGRT) approach to retrieve sperm from the testes or infuse stem cells into the testes of men with azoospermia.

Methods

Ultrasound was used to guide insertion of a 25-gauge hypodermic needle through the base of the scrotum and into the rete testis space that is contiguous with all seminiferous tubules. Medium was infused into the rete testis and aspirated to retrieve sperm from monkeys and men (NCT03291522) with azoospermia. A peripubertal patient with osteosarcoma of the femur cryopreserved a

SUBMITTER: Zielen AC 

PROVIDER: S-EPMC12071507 | biostudies-literature | 2025 Mar

REPOSITORIES: biostudies-literature