The Combination of Start-Codon-Targeted (SCoT) and <i>Falling Stone</i> (<i>FaSt</i>) Transposon-Specific Primers Provides an Efficient Marker Strategy for <i>Prunus</i> Species.

ABSTRACT: A novel primer (FaSt-R) targeting the Prunus-specific Falling Stone (FaSt) non-autonomous transposon was combined with start-codon-targeted (SCoT) primers to assess genetic diversity in 12 cultivars from six Prunus species and 28 cultivars of European plum. Compared to SCoT-only analyses, the SCoT-FaSt combination produced fewer total bands but a higher percentage of polymorphic bands, while maintaining comparable values for polymorphism information content, resolving power, gene diversity, and Shannon's index. SCoT-FaSt markers generated bands across a broader size range, which made gel patterns less dense, enabling the more accurate detection of differentially amplified fragments. Neighbor-joining and principal component analyses confirmed that SCoT-FaSt marke

SUBMITTER: Ivanovska B 

PROVIDER: S-EPMC12071656 | biostudies-literature | 2025 Apr

REPOSITORIES: biostudies-literature