The apical mucus layer alters the pharmacological properties of the airway epithelium.

ABSTRACT: Electrogenic transepithelial ion transport can be measured with the short-circuit current technique. Such experiments are frequently used to evaluate the activity of the cystic fibrosis transmembrane conductance regulator (CFTR), a cAMP-activated chloride channel that is defective in cystic fibrosis, one of the most frequent genetic diseases. Typically, CFTR activity is estimated from the effect of CFTR_inh-172, a selective CFTR inhibitor. Unexpectedly, we found that CFTR_inh-172, in addition to PPQ-102, another CFTR inhibitor, caused only partial inhibition of CFTR function, particularly in epithelia in pro-inflammatory conditions, which are characterized by abundant mucus secretion. We hypothesized that the mucus layer was responsible for the poor activity of CFTR inh

SUBMITTER: Guidone D 

PROVIDER: S-EPMC12072236 | biostudies-literature | 2025 May

REPOSITORIES: biostudies-literature